Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Identification and Characterization of CD133 pos Subpopulation Cells From a Human Laryngeal Cancer Cell Line

doi: 10.12659/MSM.895645

Figure Lengend Snippet: Expression of CD133 in Hep-2 cell lines. Flow cytometry analysis of CD133 in Hep-2cell lines. Data are representative of 3 independent experiments.

Article Snippet: Laryngeal cancer cell line Hep-2 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China); double-antibody RPMI1640 culture medium and fetal bovine serum (FBS) were obtained from GibcoTM (Invitrogen Co, Carlsbad, CA, USA); 0.25% trypsin was from TaKaRa (Dalian, China); immunomagnetic beads CD133, MTT, DMSO, and paraformaldehyde were purchased from Sigma (St. Louis, MO, USA); CD133 antibody was obtained from GeneTex (San Antonio, TX, USA); serum-free medium (SFM) is RPMI1640 culture medium with epidermal growth factor (EGF), basic fibroblast growth factor (bEGF), and insulin, which was also from GibcoTM (Invitrogen Co, Carlsbad, CA, USA).

Techniques: Expressing, Flow Cytometry

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Identification and Characterization of CD133 pos Subpopulation Cells From a Human Laryngeal Cancer Cell Line

doi: 10.12659/MSM.895645

Figure Lengend Snippet: Hep-2 cells isolation situation. Purity of CD133 positive cells were confirmed after magnetic cell sorting assessed by flow cytometry. Data are representative of triplicate experiments.

Article Snippet: Laryngeal cancer cell line Hep-2 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China); double-antibody RPMI1640 culture medium and fetal bovine serum (FBS) were obtained from GibcoTM (Invitrogen Co, Carlsbad, CA, USA); 0.25% trypsin was from TaKaRa (Dalian, China); immunomagnetic beads CD133, MTT, DMSO, and paraformaldehyde were purchased from Sigma (St. Louis, MO, USA); CD133 antibody was obtained from GeneTex (San Antonio, TX, USA); serum-free medium (SFM) is RPMI1640 culture medium with epidermal growth factor (EGF), basic fibroblast growth factor (bEGF), and insulin, which was also from GibcoTM (Invitrogen Co, Carlsbad, CA, USA).

Techniques: Isolation, FACS, Flow Cytometry

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Identification and Characterization of CD133 pos Subpopulation Cells From a Human Laryngeal Cancer Cell Line

doi: 10.12659/MSM.895645

Figure Lengend Snippet: Growth curve of CD133 + cells and CD133 − cells. Cell proliferation assay was performed by MTT analysis. Data are shown as mean ±SD and P<0.05 was considered as statistical significance.

Article Snippet: Laryngeal cancer cell line Hep-2 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China); double-antibody RPMI1640 culture medium and fetal bovine serum (FBS) were obtained from GibcoTM (Invitrogen Co, Carlsbad, CA, USA); 0.25% trypsin was from TaKaRa (Dalian, China); immunomagnetic beads CD133, MTT, DMSO, and paraformaldehyde were purchased from Sigma (St. Louis, MO, USA); CD133 antibody was obtained from GeneTex (San Antonio, TX, USA); serum-free medium (SFM) is RPMI1640 culture medium with epidermal growth factor (EGF), basic fibroblast growth factor (bEGF), and insulin, which was also from GibcoTM (Invitrogen Co, Carlsbad, CA, USA).

Techniques: Proliferation Assay

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Identification and Characterization of CD133 pos Subpopulation Cells From a Human Laryngeal Cancer Cell Line

doi: 10.12659/MSM.895645

Figure Lengend Snippet: Colony formation assay of sorted CD133 + cells and CD133 − cells. Photographs are representative of triplicate experiments. ( A ) CD133 + cells; ( B ) CD133 − cells.

Article Snippet: Laryngeal cancer cell line Hep-2 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China); double-antibody RPMI1640 culture medium and fetal bovine serum (FBS) were obtained from GibcoTM (Invitrogen Co, Carlsbad, CA, USA); 0.25% trypsin was from TaKaRa (Dalian, China); immunomagnetic beads CD133, MTT, DMSO, and paraformaldehyde were purchased from Sigma (St. Louis, MO, USA); CD133 antibody was obtained from GeneTex (San Antonio, TX, USA); serum-free medium (SFM) is RPMI1640 culture medium with epidermal growth factor (EGF), basic fibroblast growth factor (bEGF), and insulin, which was also from GibcoTM (Invitrogen Co, Carlsbad, CA, USA).

Techniques: Colony Assay

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Identification and Characterization of CD133 pos Subpopulation Cells From a Human Laryngeal Cancer Cell Line

doi: 10.12659/MSM.895645

Figure Lengend Snippet: Tumor weight, volume and forming time among three groups (mean ±SD).

Article Snippet: Laryngeal cancer cell line Hep-2 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China); double-antibody RPMI1640 culture medium and fetal bovine serum (FBS) were obtained from GibcoTM (Invitrogen Co, Carlsbad, CA, USA); 0.25% trypsin was from TaKaRa (Dalian, China); immunomagnetic beads CD133, MTT, DMSO, and paraformaldehyde were purchased from Sigma (St. Louis, MO, USA); CD133 antibody was obtained from GeneTex (San Antonio, TX, USA); serum-free medium (SFM) is RPMI1640 culture medium with epidermal growth factor (EGF), basic fibroblast growth factor (bEGF), and insulin, which was also from GibcoTM (Invitrogen Co, Carlsbad, CA, USA).

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Identification and Characterization of CD133 pos Subpopulation Cells From a Human Laryngeal Cancer Cell Line

doi: 10.12659/MSM.895645

Figure Lengend Snippet: Sensitivity of CD133 + cells and CD133 − cells to chemotherapy agents. MTT analysis is detected after 48 h of culture in the presence of 5, 10, 15, and 20 μM DDP. Data are expressed as mean ±SD and P<0.05 was considered as statistical significance.

Article Snippet: Laryngeal cancer cell line Hep-2 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China); double-antibody RPMI1640 culture medium and fetal bovine serum (FBS) were obtained from GibcoTM (Invitrogen Co, Carlsbad, CA, USA); 0.25% trypsin was from TaKaRa (Dalian, China); immunomagnetic beads CD133, MTT, DMSO, and paraformaldehyde were purchased from Sigma (St. Louis, MO, USA); CD133 antibody was obtained from GeneTex (San Antonio, TX, USA); serum-free medium (SFM) is RPMI1640 culture medium with epidermal growth factor (EGF), basic fibroblast growth factor (bEGF), and insulin, which was also from GibcoTM (Invitrogen Co, Carlsbad, CA, USA).

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Identification and Characterization of CD133 pos Subpopulation Cells From a Human Laryngeal Cancer Cell Line

doi: 10.12659/MSM.895645

Figure Lengend Snippet: Tumor weight, volume and forming time among three groups under DDP treatment (mean ±SD).

Article Snippet: Laryngeal cancer cell line Hep-2 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China); double-antibody RPMI1640 culture medium and fetal bovine serum (FBS) were obtained from GibcoTM (Invitrogen Co, Carlsbad, CA, USA); 0.25% trypsin was from TaKaRa (Dalian, China); immunomagnetic beads CD133, MTT, DMSO, and paraformaldehyde were purchased from Sigma (St. Louis, MO, USA); CD133 antibody was obtained from GeneTex (San Antonio, TX, USA); serum-free medium (SFM) is RPMI1640 culture medium with epidermal growth factor (EGF), basic fibroblast growth factor (bEGF), and insulin, which was also from GibcoTM (Invitrogen Co, Carlsbad, CA, USA).

Techniques: